Effect of polybrene on isolation of Chlamydia trachomatis from clinical specimens.

نویسندگان

  • P Sankar-Mistry
  • V Albota
  • B Knelsen
چکیده

Polybrene treatment of McCoy cells was used to increase the infectivity of Chlamydia trachomatis in vitro. The isolation rate for C. trachomatis in 500 specimens from males and females attending a sexually transmitted disease clinic and other clinics increased by 6.8% with the Polybrene treatment. The data also suggested that this treatment facilitated the diagnosis of low grade or latent infections.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genotyping of Chlamydia trachomatis from endocervical specimens in Shiraz, Iran

The objective of this study was to determine the prevalence and distribution of Chlamydia trachomatisgenotypes in Shiraz, Iran. Two hundred twelve cervical swab samples were collected from women attendingShahid Motahari Polyclinic in Shiraz, Iran. The endocervical specimens were screened for C. trachomatis byplasmid PCR. Genotyping was performed in C. trachomatis-positive samples by nested PCR ...

متن کامل

Improved method for isolation and growth of Chlamydia trachomatis in McCoy cells treated with cycloheximide using polyethylene glycol.

The sensitivity of non-replicating McCoy cells pretreated with polyethylene glycol for the isolation of Chlamydia trachomatis from clinical specimens and for the growth of a laboratory strain was compared with the sensitivity of untreated non-replicating cell cultures. The concentration of polyethylene glycol in different solutions and the time of addition to the cell culture medium were critic...

متن کامل

Micro direct inoculation method for the isolation and identification of Chlamydia trachomatis.

Current standard procedures for the isolation and identification of Chlamydia trachomatis are laborious. A more rapid method for the isolation and identification of C. trachomatis has been developed. This method features the use of a multiwell microplate to which trypsinized McCoy cells and specimens are added simultaneously, followed by centrifugation. A simplified one-step iodine-glycerol sta...

متن کامل

Low Levels of Chlamydia Trachomatis Isolation from Patients of Obstetrics and Midwifery in Saudi Arabia

کلامیدیا یکی از علل عمده عفونت های ادراری - تناسلی در سراسر دنیا می باشد. عفونت ناشی از این ارگانیسم به طور متداول در کلینک های زنان و مامایی یافت می شود. جداسازی بوسیله کشت بافت، طریق استاندارد و بسیار حساسی جهت اثبات این عفونت می باشد هر چند که توسط روش های مستقیم تعیین آنتی ژن با استفاده از ایمونوفلورسنت یا تعیین عیار آنزیم سرمی و اخیراً نیز بوسیله بررسی ژنتیکی با تکنیک واکنش زنجیره پلیمراز ت...

متن کامل

Improved isolation of Chlamydia trachomatis from a low-prevalence population by using polyethylene glycol.

The effect of polyethylene glycol (PEG) on the isolation of Chlamydia trachomatis was evaluated in our laboratory. Initial range-finding experiments demonstrated that the number of chlamydial inclusion bodies increased with increasing PEG concentrations. However, PEG concentrations above 10.5% became progressively more toxic to the McCoy cell monolayers. When 50 frozen clinical Chlamydia isolat...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 22 4  شماره 

صفحات  -

تاریخ انتشار 1985